The HUMAN (Actin) Peptide Pool consists of 92 overlapping peptides derived from a peptide scan through Actin, alpha skeletal muscle (UniProt ID: P68133) of Homo sapiens. The peptides are 15mers that overlap by 11 amino acids.

One unit of HUMAN (Actin) Peptide Pool allows the stimulation of 2,5 x 10⁸ cells.

Actin is a ubiquitously expressed protein and therefore an excellent negative control for T cell assays. Relevant controls are critical for measuring antigen-specific responses as they allow accurate interpretation of test results. The HUMAN (Actin) Peptide Pool can rule out non-specific binding of peptides to the tested T cells, while a negative control with medium detects general background signals.

Examples of publications where actin was used as a negative control:

• Baker FL, et al.: Exercise mobilizes diverse antigen specific T-cells and elevates neutralizing antibodies in humans with natural immunity to SARS CoV-2. Brain Behav Immun Health.
  2023 Mar;28:100600. doi: 10.1016/j.bbih.2023.100600.

• Sweeney, E et al.: Photothermal Prussian blue nanoparticles generate potent multi‐targeted tumor‐specific T cells as an adoptive cell therapy.
  Bioeng Transl Med. 2023;e10639. doi.org/10.1002/btm2.10639.

• Roy, P et al.: Immunodominant MHC-II (Major Histocompatibility Complex II) Restricted Epitopes in Human Apolipoprotein B. Circ Res. 2022 Jul 22;131(3):258-276.
  doi: 10.1161/CIRCRESAHA.122.321116.

• Lasagna, Angioletta et al. “The immunogenicity and the safety of the adjuvanted glycoprotein E (gE)-based recombinant vaccine against herpes zoster (RZV) in cancer patients during immunotherapy.”
  Human vaccines & immunotherapeutics vol. 19,3 (2023): 2288282. doi:10.1080/21645515.2023.2288282