Pool of 110 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through Wilms tumor protein (WT33) (Swiss-Prot ID: P19544) of Homo sapiens (Human) for T cell assays (e.g. ELISPOT).
One unit allows the stimulation of 2,5 x 108 cells.
15 nmol (approx. 25µg)/peptide for stimulation of 2,5 x 108 cells
Dissolve in a minimum amount of pure DMSO (approx. 40μl) and dilute with water to the desired concentration. Please pay attention that the final concentration of DMSO must be below 1% (v/v) to avoid toxicity in the biological
Store dry at -20°C, avoid repeated thawing and freezing
Each peptide ESI-MS checked, pool is purified by solid phase extraction