Pool of 326 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through Tetanus toxin (UniProt ID: P04958) of Clostridium tetani (strain Massachusetts / E88) for T cell assays (e.g. ELISPOT)
One unit allows the stimulation of 2,5 x 108 cells.
18 nmol (approx. 30µg)/peptide for stimulation of 2,5 x 108 cells
Dissolve in a minimum amount of pure DMSO (approx. 40μl) and dilute with water to the desired concentration. Please pay attention that the final concentration of DMSO must be below 1% (v/v) to avoid toxicity in the biological
Store dry at -20°C, avoid repeated thawing and freezing
Each peptide ESI-MS checked, pool is purified by solid phase extraction