Product information "HCoV-229E (Spike Glycoprotein) Peptide Pool"
Pool of 291 overlapping peptides (delivered in two subpools of 146 & 145 peptides) derived from a peptide scan (15mers with 11 aa overlap) through Spike glycoprotein (UniProt: P15423) of Human coronavirus 229E (HCoV-229E) for T cell assays (e.g. ELISPOT).
One unit allows the stimulation of 2,5 x 108 cells.
15 nmol (approx. 25µg)/peptide for stimulation of 2,5 x 108 cells
Dissolve in a minimum amount of pure DMSO (approx. 40μl) and dilute with water to the desired concentration. Please pay attention that the final concentration of DMSO must be below 1% (v/v) to avoid toxicity in the biological
Store dry at -20°C, avoid repeated thawing and freezing
Each peptide ESI-MS checked, pool is purified by solid phase extraction