EBV BARF1 Peptide Pool
Pool of 53 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through Secreted protein BARF1 (UniProt: P03225) of Homo sapiens Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4).
One unit allows the stimulation of 2,5 x 108 cells.
For long-term storage, we recommend to store the peptides in lyophilized form at -20°C, or preferably at -80°C in sealed containers to minimize peptide degradation. Under these conditions, peptides can be stored for up to several years and this prevents different kinds of degradation such as oxidation, formation of secondary structures and bacterial contamination. The shelf stability of peptides is sequence-dependent. Sequences containing cysteine, methionine, tryptophan, asparagine, glutamine and N-terminal glutamic acid will have a shorter shelf life than other peptides. For short-time storage, we recommend to store the peptides in a refrigerator (+4°C). The peptides should be protected from direct sunlight and peptides with fluorophores should be stored in darkness.
Before opening the peptides, it is better to equilibrate to room temperature as peptides tend to be hygroscopic and can lead to cause condensation. Condensation can reduce the stability of the peptides. Before using the peptides, we recommend centrifuging them.
Dissolved peptides are less stable than lyophilized peptides and shouldn’t be stored long-term. We recommend to lyophilize the dissolved peptides.
Unfortunately, it is not possible to predict if a peptide is water-soluble by studying its structure alone, but there are clues for determining solubility, especially for short peptides. The ε-amino group of Lysine and the guanidine group of Arginine are in pronated form in peptides sold as TFA salts and are easily dissolved in water. Peptides containing many acidic amino acids such as aspartic acid and glutamic acid, however, are difficult to dissolve in water but are readily soluble with ammonia or basic buffers.
Here you will find certain basic characteristics to predict solubility:
- Short peptides with <5 amino acids are usually highly soluble in aqueous solutions. However, if the peptides contain only hydrophobic amino acids, they may be difficult or impossible to dissolve in water.
- Peptides with many hydrophilic amino acids (>25%) (E,D,K,R,H) and few hydrophobic amino acids (<25%) are usually well soluble in aqueous solutions.
- Peptides containing ≥50% hydrophobic amino acids should be well or partially soluble in aqueous solutions. If the peptides are only partially soluble, organic solvents such as DMSO, DMF, acetonitrile, isopropyl alcohol, ethanol, acetic acid, 4-8 M guanidine hydrochloride (GdnHCl), or urea should be used and further diluted with aqueous solutions. However, peptides containing C, M or W should not be dissolved in DMSO due to oxidation reactions.
- Hydrophobic peptides with more than 75% hydrophobic amino acids are usually not soluble in water. For these peptides, strong solvents such as TFA or formic acid should be used for dissolution. Dilution with an aqueous solution could again lead to precipitation of the peptide. Therefore, high concentrations of organic solvents or denaturants may be necessary to completely dissolve the peptide.
- Peptides containing a very high proportion (>75%) of the following amino acids: D, E, H, K, N, Q, R, S, T, or Y can form intermolecular hydrogen bonds, which leads to gel formation in aqueous solutions. Such peptides are best dissolved with organic solvents. Dilution with aqueous solutions should only be done dropwise. The limit of aqueous solubility can be recognized by the turbidity of the solution.
| Gene: | BARF1 | |
| Delivery: | overnight | |
| Counter Ion: | TFA | |
| Protein: | Secreted protein BARF1 | |
| UniProt Id: | P03228 | |
| Species: | Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4) |
Protocols and Tips
Data sheets
Safety data sheet poly peptides:Bosc-Bierne, G et al. „Efficient Quality Control of Peptide Pools by UHPLC and Simultaneous UV and HRMS Detection. Separations 2024, 11, 156. https://doi.org/10.3390/separations11050156
Lin, Yusheng et al. “VISTA drives macrophages towards a pro-tumoral phenotype that promotes cancer cell phagocytosis yet down-regulates T cell responses.” Experimental hematology & oncology vol. 13,1 35. 29 Mar. 2024, doi:10.1186/s40164-024-00501-x
Kim, Sang-Hoon et al. “Omicron BA.2 breakthrough infection elicits CD8+ T cell responses recognizing the spike of later Omicron subvariants.” Science immunology vol. 9,91 (2024): eade6132. doi:10.1126/sciimmunol.ade6132
€201.25*
sterile and endotoxin free
Delivery Format: The product is supplied freeze dried.
Controls
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