Influenza A (H1N1) HA Peptide Pool

Description

Accelerate T-cell immunomonitoring and cross-strain vaccine research with complete HA1/HA2 sequence coverage.

The Influenza A (H1N1) Hemagglutinin (HA) Peptide Pool is a high-precision antigen screening tool designed to evaluate CD4+ and CD8+ T-cell immunity. Derived from the full-length Hemagglutinin protein(UniProt ID: C3W5S1) of Influenza A virus (strain swl A/California/04/2009 H1N1), this overlapping peptide pool (15mers with 11 aa overlap) provides coverage of both the variable HA1 globular head and the highly conserved HA2 stalk domain.

This catalog peptide pool consists of 139 high-quality synthetic peptides tailored for robust in vitro and ex vivo recall assays, profiling donor PBMCs, monitoring vaccine efficacy, or deconvoluting T-cell receptor (TCR) specificities.

Key Features & Benefits

• Complete Antigenic Coverage: Overlapping peptide design ensures comprehensive mapping of both linear CD4+ and MHC Class I-restricted CD8+ epitopes without sequence gaps.
• Target Conserved & Variable Regions: Ideal for identifying cross-protective T-cell responses directed against the conserved HA2 stalk alongside strain-specific HA1 targets.
• Optimized for High-Throughput Assays: Formulated for reliable performance in ELISpot, ICS (Intracellular Cytokine Staining), Fluorospot, and flow-cytometric MHC multimer staining.
• High Batch-to-Batch Reproducibility: Synthesized under rigorous quality controls by peptides&elephants to guarantee consistent quality, solubility, and assay performance.

Applications

• Ex Vivo Immunomonitoring: Quantify functional T-cell memory (IFN-gamma, IL-2, TNF-alpha) in human donor samples or animal models.
• Vaccine Candidate Evaluation: Screen T-cell responses elicited by seasonal H1N1 or broad-spectrum universal flu vaccine constructs.
• Epitope Discovery & Deconvolution: Serves as a master screening panel before deconvoluting individual HLA-restricted epitopes.

One unit allows the stimulation of 2,5 x 10⁸ cells.