Tetanus Toxin 1273-1284 Peptide GQIGNDPNRDIL

The Clostridium tetani Peptide is a single peptide for stimulation of antigen-specific T cells in T cell assays such as ELISPOT, ICS, cytotoxicity or proliferation assays.

The Clostridium tetani Peptide is a single peptide for stimulation of antigen-specific T cells in T cell assays such as ELISPOT, ICS, cytotoxicity or proliferation assays.

A universal CD4+ T-cell helper epitope and gold-standard positive control for human immunomonitoring and vaccine design.The Tetanus Toxin 830–843 peptide (QYIKANSKFIGITE, epitope ID: 52929), commonly referred to as the P2 helper peptide, is one of the most extensively characterized immunodominant T-cell epitopes in immunology. Derived from the heavy chain of Clostridium tetani (strain Massachusetts / E88) neurotoxin (UniProt: P04958), this 14-mer sequence represents a primary T-cell target of vaccine-induced memory following standard Tetanus Toxoid immunization. Because tetanus vaccination is widely implemented, virtually all human donors harbour stable, long-term memory T cells against this sequence, making it an indispensable control for assay validation and conjugate vaccine development.Key Features & BenefitsPromiscuous HLA Class II Binding: Binds across a broad range of HLA-DR alleles: HLA-DRB1*01:01, HLA-DRB1*07:01, HLA-DRB1*11:01, HLA-DRB1*03:01, HLA-DRB5*01:01, HLA-DRB1*13:02 and more.Extensively Validated: Backed by over 40 scientific publications and validated in dozens of T-cell, B-cell, and MHC ligand assays worldwide.Gold-Standard Positive Control: Reliable recall antigen for evaluating functional T-cell viability (IFN-gamma, IL-2, TNF-alpha) in human peripheral blood mononuclear cells (PBMCs).Complementary T-Helper Target: Frequently paired or benchmarked alongside the P30 epitope (TT 947–967) to provide robust, promiscuous CD4+ T-cell help in synthetic vaccine design, conjugate constructs, and multi-epitope recall panels.High Chemical Quality: peptides&elephants' quality standards ensure exceptional quality, lot-to-lot consistency, and reliable assay performance.ApplicationsPBMC Quality Control & Recall Assays: Serves as a robust positive control in ELISpot, Fluorospot, Intracellular Cytokine Staining (ICS), and proliferation assays.T-Cell Helper Sequence for Conjugates: Widely utilized as a universal P2 helper T-cell epitope incorporated into synthetic peptide vaccines, neoantigen constructs, and carbohydrate-protein conjugates to boost immunogenicity.MHC Class II Binding Kinetics: Ideal reference ligand for HLA-DR competitive binding and peptide exchange assays.

A universal CD4+ T-cell helper epitope and promiscuous MHC Class II control for human immunomonitoring and conjugate vaccine design.The Tetanus Toxin 947–967 peptide (FNNFTVSFWLRVPKVSASHLE, epitope ID: 17207), widely known as the P30 helper epitope, is one of the most prominent promiscuous T-cell epitopes in cellular immunology. Derived from the heavy chain of Clostridium tetani (strain Massachusetts / E88) neurotoxin (UniProt: P04958), this 21-mer sequence represents a core CD4+ T-cell target of vaccine-induced immune memory established by widespread Tetanus Toxoid vaccination. Because of its broad binding capacity across diverse human MHC Class II molecules, P30 is an essential reference tool for PBMC recall assays, immunogenicity enhancement, and vaccine carrier design. Key Features & Benefits Promiscuous HLA Class II Binding: Binds efficiently across a wide spectrum of human MHC Class II alleles, ensuring high population coverage without donor HLA restriction.Extensively Validated: Backed by dozens of scientific publications and validated across nearly 100 T-cell assays, B-cell assays, and MHC ligand binding studies worldwide.Robust Positive Control: Serves as a gold-standard recall antigen for evaluating functional CD4+ T-cell viability (IFN-gamma, IL-2, TNF-alpha) in human peripheral blood mononuclear cells (PBMCs).Ideal Carrier & Helper Sequence: Frequently paired or benchmarked alongside the P2 epitope (TT 830–843) to enhance the immunogenicity of synthetic peptide vaccines, neoantigens, and carbohydrate conjugates.High Chemical Quality: The rigorous quality standards of peptides&elephants guarantee exceptional batch-to-batch consistency, solubility, and assay reproducibility. Applications PBMC Quality Control & Recall Assays: Acts as a reliable positive control in ELISpot, Fluorospot, Intracellular Cytokine Staining (ICS), and proliferation assays evaluating Tetanus Toxoid-induced T-cell memory.Conjugate Vaccine & Neoantigen Design: Widely incorporated into synthetic multi-epitope construct designs to provide strong, promiscuous CD4+ T-cell help for antibody or CD8+ T-cell responses.HLA Class II Profiling: Excellent reference target for investigating CD4+ T-cell receptor (TCR) cross-reactivity and MHC-II binding kinetics.

Peptide pool of 326 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through Tetanus toxin (UniProt ID: P04958) of Clostridium tetani (strain Massachusetts / E88) for T cell assays (e.g. ELISPOT) One unit allows the stimulation of 2,5 x 108 cells.