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CEF control Peptide Pool (>95% HPLC, advanced)



The CEF peptide pool is a lyophilized mixture of 32 peptides from cytomegalovirus (CMV), Epstein-Barr virus (EBV), and influenza virus. It consists of defined HLA class I-restricted T cell epitopes from these three viruses, and can be used as a positive control to stimulate T cells. The CEF peptide pool is used as a positive control to stimulate T cells. It induces secretion of e.g., IFN-γ, IL-2, and granzyme B from human CD8 T cells.

One unit allows the stimulation of 2,5 x 108 cells.

These are sequence, source and corresponding MHC class I molecule of each peptide:

Sequence Source MHC class I molecule
H-VSDGGPNLY-OH Influenza A HLA-A1
H-CTELKLSDY-OH Influenza A HLA-A1
H-GILGFVFTL-OH Influenza M HLA-A2
H-FMYSDFHFI-OH Influenza A HLA-A2
H-CLGGLLTMV-OH EBV LMP2A HLA-A2
H-GLCTLVAML-OH EBV BMLF1 HLA-A2
H-NLVPMVATV-OH HCMV pp65 HLA-A2
H-KTGGPIYKR-OH Influenza NP HLA-A68
H-RVLSFIKGTK-OH Influenza NP HLA-A3
H-ILRGSVAHK-OH Influenza A HLA-A3
H-RVRAYTYSK-OH EBV HLA-A3
H-RLRAEAQVK-OH EBV HLA-A3
H-SIIPSGPLK-OH Influenza M HLA-A3/A11/A6
H-AVFDRKSDAK-OH EBV EBNA 4NP HLA-A11
H-IVTDFSVIK-OH EBV HLA-A11
H-ATIGTAMYK-OH EBV HLA-A11
H-DYCNVLNKEF-OH EBV RTA HLA-A24
H-LPFDKTTVM-OH Influenza NP HLA-B7
H-RPPIFIRRL-OH EBV  HLA-B7
H-ELRSRYWAI-OH   Influenza NP HLA-B8
H-RAKFKQLL-OH   EBV B2LF-1 HLA-B8
H-FLRGRAYGL-OH   EBV EBNA 3A HLA-B8
H-QAKWRLQTL-OH   EBV EBNA 3 HLA-B8
H-SDEEEAIVAYTL-OH   HCMV HLA-B18
H-SRYWAIRTR-OH   Influenza NP HLA-B27
H-ASCMGLIY-OH  Influenza A  HLA-B27
H-RRIYDLIEL-OH   EBV EBNA 3C HLA-B27
H-YPLHEQHGM-OH  EBV EBNA 3A  HLA-B35
H-IPSINVHHY-OH   CMV pp65 HLA-B35
H-EENLLDFVRF-OH   EBV HLA-B44
H-EFFWDANDIY-OH  HCMV  HLA-B44
H-TPRVTGGGAM-OH   HCMV HLA-B7

For more information and application examples, read our blog posts:

Gene:n/a
Delivery:overnight
Counter Ion:TFA
Protein:Cytomegalovirus, Epstein- Barr virus and Influenza virus
UniProt Id:n/a
Species:Cytomegalovirus, Epstein- Barr virus and Influenza virus
Application :T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response
Indication :Control
Amount/Aliquote :15 nmol (approx. 25µg)/peptide for stimulation of 2,5 x 108 cells
Bosc-Bierne G, Ewald S, Kreuzer OJ, Weller MG. Efficient Quality Control of Peptide Pools by UHPLC and Simultaneous UV and HRMS Detection. Separations. 2024; 11(5):156. https://doi.org/10.3390/separations11050156

Garcia-Marquez MA, Thelen M, Bauer E, et al.: Germline homozygosity and allelic imbalance of HLA-I are common in esophagogastric adenocarcinoma and impair the repertoire of immunogenic peptides. Journal for ImmunoTherapy of Cancer 2024;12:e007268. doi: 10.1136/jitc-2023-007268

€121.00*

Delivery time 2-3 days
sterile and endotoxin free
Delivery Format: The product is supplied freeze dried.
Product number: LB01359