CEF control Peptide Pool (>95% HPLC, advanced)
The CEF peptide pool is a lyophilized mixture of 32 peptides from cytomegalovirus (CMV), Epstein-Barr virus (EBV), and influenza virus. It consists of defined HLA class I-restricted T cell epitopes from these three viruses, and can be used as a positive control to stimulate T cells. The CEF peptide pool is used as a positive control to stimulate T cells. It induces secretion of e.g., IFN-γ, IL-2, and granzyme B from human CD8 T cells.
One unit allows the stimulation of 2,5 x 108 cells.These are sequence, source and corresponding MHC class I molecule of each peptide:
Sequence | Source | MHC class I molecule |
H-VSDGGPNLY-OH | Influenza A | HLA-A1 |
H-CTELKLSDY-OH | Influenza A | HLA-A1 |
H-GILGFVFTL-OH | Influenza M | HLA-A2 |
H-FMYSDFHFI-OH | Influenza A | HLA-A2 |
H-CLGGLLTMV-OH | EBV LMP2A | HLA-A2 |
H-GLCTLVAML-OH | EBV BMLF1 | HLA-A2 |
H-NLVPMVATV-OH | HCMV pp65 | HLA-A2 |
H-KTGGPIYKR-OH | Influenza NP | HLA-A68 |
H-RVLSFIKGTK-OH | Influenza NP | HLA-A3 |
H-ILRGSVAHK-OH | Influenza A | HLA-A3 |
H-RVRAYTYSK-OH | EBV | HLA-A3 |
H-RLRAEAQVK-OH | EBV | HLA-A3 |
H-SIIPSGPLK-OH | Influenza M | HLA-A3/A11/A6 |
H-AVFDRKSDAK-OH | EBV EBNA 4NP | HLA-A11 |
H-IVTDFSVIK-OH | EBV | HLA-A11 |
H-ATIGTAMYK-OH | EBV | HLA-A11 |
H-DYCNVLNKEF-OH | EBV RTA | HLA-A24 |
H-LPFDKTTVM-OH | Influenza NP | HLA-B7 |
H-RPPIFIRRL-OH | EBV | HLA-B7 |
H-ELRSRYWAI-OH | Influenza NP | HLA-B8 |
H-RAKFKQLL-OH | EBV B2LF-1 | HLA-B8 |
H-FLRGRAYGL-OH | EBV EBNA 3A | HLA-B8 |
H-QAKWRLQTL-OH | EBV EBNA 3 | HLA-B8 |
H-SDEEEAIVAYTL-OH | HCMV | HLA-B18 |
H-SRYWAIRTR-OH | Influenza NP | HLA-B27 |
H-ASCMGLIY-OH | Influenza A | HLA-B27 |
H-RRIYDLIEL-OH | EBV EBNA 3C | HLA-B27 |
H-YPLHEQHGM-OH | EBV EBNA 3A | HLA-B35 |
H-IPSINVHHY-OH | CMV pp65 | HLA-B35 |
H-EENLLDFVRF-OH | EBV | HLA-B44 |
H-EFFWDANDIY-OH | HCMV | HLA-B44 |
H-TPRVTGGGAM-OH | HCMV | HLA-B7 |
For more information and application examples, read our blog posts:
Gene: | n/a | |
Delivery: | overnight | |
Counter Ion: | TFA | |
Protein: | Cytomegalovirus, Epstein- Barr virus and Influenza virus | |
UniProt Id: | n/a | |
Species: | Cytomegalovirus, Epstein- Barr virus and Influenza virus | |
Application : | T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response | |
Indication : | Control | |
Amount/Aliquote : | 15 nmol (approx. 25µg)/peptide for stimulation of 2,5 x 108 cells |
Protocols and Tips
Data sheets
Safety data sheet poly peptides:
Bosc-Bierne G, Ewald S, Kreuzer OJ, Weller MG. Efficient Quality Control of Peptide Pools by UHPLC and Simultaneous UV and HRMS Detection. Separations. 2024; 11(5):156. https://doi.org/10.3390/separations11050156
Garcia-Marquez MA, Thelen M, Bauer E, et al.: Germline homozygosity and allelic imbalance of HLA-I are common in esophagogastric adenocarcinoma and impair the repertoire of immunogenic peptides. Journal for ImmunoTherapy of Cancer 2024;12:e007268. doi: 10.1136/jitc-2023-007268
Garcia-Marquez MA, Thelen M, Bauer E, et al.: Germline homozygosity and allelic imbalance of HLA-I are common in esophagogastric adenocarcinoma and impair the repertoire of immunogenic peptides. Journal for ImmunoTherapy of Cancer 2024;12:e007268. doi: 10.1136/jitc-2023-007268
€121.00*
Delivery time 2-3 days
sterile and endotoxin free
Delivery Format: The product is supplied freeze dried.
sterile and endotoxin free
Delivery Format: The product is supplied freeze dried.
Product number:
LB01359