HUMAN (Actin) Peptide Pool
Prices plus VAT plus shipping costs
Delivery time overnight
sterile and endotoxin free
Delivery Format: The product is supplied freeze dried.
- Order number: LB01856
The HUMAN (Actin) Peptide Pool consists of 92 overlapping peptides derived from a peptide scan through Actin, alpha skeletal muscle (UniProt ID: P68133) of Homo sapiens. The peptides are 15mers that overlap by 11 amino acids.
One unit of HUMAN (Actin) Peptide Pool allows the stimulation of 2,5 x 108 cells.
Actin is a ubiquitously expressed protein and therefore an excellent negative control for T cell assays. Relevant controls are critical for measuring antigen-specific responses as they allow accurate interpretation of test results. The HUMAN (Actin) Peptide Pool can rule out non-specific binding of peptides to the tested T cells, while a negative control with medium detects general background signals.
Examples of publications where actin was used as a negative control:
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Baker FL, et al.: Exercise mobilizes diverse antigen specific T-cells and elevates neutralizing antibodies in humans with natural immunity to SARS CoV-2. Brain Behav Immun Health.
2023 Mar;28:100600. doi: 10.1016/j.bbih.2023.100600. -
Sweeney, E et al.: Photothermal Prussian blue nanoparticles generate potent multi‐targeted tumor‐specific T cells as an adoptive cell therapy.
Bioeng Transl Med. 2023;e10639. doi.org/10.1002/btm2.10639. -
Roy, P et al.: Immunodominant MHC-II (Major Histocompatibility Complex II) Restricted Epitopes in Human Apolipoprotein B. Circ Res. 2022 Jul 22;131(3):258-276.
doi: 10.1161/CIRCRESAHA.122.321116. -
Lasagna, Angioletta et al. “The immunogenicity and the safety of the adjuvanted glycoprotein E (gE)-based recombinant vaccine against herpes zoster (RZV) in cancer patients during immunotherapy.”
Human vaccines & immunotherapeutics vol. 19,3 (2023): 2288282. doi:10.1080/21645515.2023.2288282
Gene: | ACTA1 | |
No Peptides: | 92 peptides | |
Amount Peptide: | 15 nmol (approx. 25µg)/peptide for stimulation of 2,5 x 108 cells | |
Amount Aliquote: | 2,3 mg | |
Uniprot Id: | P68133 | |
Solubility: | Dissolve in a minimum amount of pure DMSO (approx. 40μl) and dilute with water to the desired concentration. Please pay attention that the final concentration of DMSO must be below 1% (v/v) to avoid toxicity in the biological | |
Storage: | Store dry at -20°C, avoid repeated thawing and freezing | |
Delivery: | overnight | |
Purity: | Each peptide ESI-MS checked, pool is purified by solid phase extraction | |
Counter Ion: | TFA | |
Protein: | Actin, alpha skeletal muscle | |
Species: | Homo sapiens (Human) | |
Application : | T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response | |
Indication : | Control |
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Data sheets