Control Peptides and Peptide Pools - p&e Shop

The linear peptide B8R 20-27, with the amino acid sequence TSYKFESV (epitope ID 66504), is a well-established H2-Kb-restricted epitope conserved among various Vaccinia virus strains. [1] It is part of the: Soluble interferon gamma receptor OPG193 (UniProt:P24770) from Vaccinia virus IFN-gamma receptor (UniProt:Q66793) from Ectromelia mousepox virus IFN-gamma receptor-like protein (UniProt:G0XTP5) from Cowpox virus The Vaccinia virus was the live vaccine used to eradicate smallpox. It belongs to the orthopoxvirus family and provides protection against a range of orthopoxviruses. Currently, the most concerning of these is the monkeypox virus, but human cowpox infections are also being reported regularly in Europe. [1] The B8R 20-27 peptide, TSYKFESV, is also contained in recombinant and non-recombinant Modified Vaccinia virus Ankara (MVA). [1] The Modified Vaccinia virus Ankara has greatly reduced virulence and can no longer replicate in most mammalian cells. [2] It is often used as a vaccine vector, due to its cost-effectiveness, safety, and high immunogenicity. Recombinant MVA vaccines can generate robust humoral and cellular immune responses against various pathogens. In vaccine development, the B8R 20-27 peptide is used as a positive control to demonstrate that a lack of response is not due to immunization failure. The vaccinia virus B8R gene encodes a homolog of the secreted IFN-γ receptor. By binding to IFN-γ, B8R neutralizes its antiviral activity and is therefore involved in virus evasion of host interferon responses. [1,3]You can read a current application example in our blog post "Vaccines against Chlamydia trachomatis: Immunogenicity analysis via peptide pool" or view the original publication [4] via the link under the References tab.

The CEF peptide pool is a lyophilized mixture of 23 peptides from cytomegalovirus (CMV), Epstein-Barr virus (EBV), and influenza virus. It consists of defined HLA class I-restricted T cell epitopes from these three viruses. The CEF peptide pool is used as a positive control to stimulate T cells. It induces secretion of e.g., IFN-γ, IL-2, and granzyme B from human CD8 T cells. The peptides of this product are supplied as trifluoracetate salts. One unit allows the stimulation of 2,5 x 108 cells.

The CEF peptide pool is a lyophilized mixture of 32 peptides from cytomegalovirus (CMV), Epstein-Barr virus (EBV), and influenza virus. It consists of defined HLA class I-restricted T cell epitopes from these three viruses, and can be used as a positive control to stimulate T cells. The CEF peptide pool is used as a positive control to stimulate T cells. It induces secretion of e.g., IFN-γ, IL-2, and granzyme B from human CD8 T cells. One unit allows the stimulation of 2,5 x 108 cells.These are sequence, source and corresponding MHC class I molecule of each peptide: Sequence Source MHC class I molecule H-VSDGGPNLY-OH Influenza A HLA-A1 H-CTELKLSDY-OH Influenza A HLA-A1 H-GILGFVFTL-OH Influenza M HLA-A2 H-FMYSDFHFI-OH Influenza A HLA-A2 H-CLGGLLTMV-OH EBV LMP2A HLA-A2 H-GLCTLVAML-OH EBV BMLF1 HLA-A2 H-NLVPMVATV-OH HCMV pp65 HLA-A2 H-KTGGPIYKR-OH Influenza NP HLA-A68 H-RVLSFIKGTK-OH Influenza NP HLA-A3 H-ILRGSVAHK-OH Influenza A HLA-A3 H-RVRAYTYSK-OH EBV HLA-A3 H-RLRAEAQVK-OH EBV HLA-A3 H-SIIPSGPLK-OH Influenza M HLA-A3/A11/A6 H-AVFDRKSDAK-OH EBV EBNA 4NP HLA-A11 H-IVTDFSVIK-OH EBV HLA-A11 H-ATIGTAMYK-OH EBV HLA-A11 H-DYCNVLNKEF-OH EBV RTA HLA-A24 H-LPFDKTTVM-OH Influenza NP HLA-B7 H-RPPIFIRRL-OH EBV  HLA-B7 H-ELRSRYWAI-OH   Influenza NP HLA-B8 H-RAKFKQLL-OH   EBV B2LF-1 HLA-B8 H-FLRGRAYGL-OH   EBV EBNA 3A HLA-B8 H-QAKWRLQTL-OH   EBV EBNA 3 HLA-B8 H-SDEEEAIVAYTL-OH   HCMV HLA-B18 H-SRYWAIRTR-OH   Influenza NP HLA-B27 H-ASCMGLIY-OH  Influenza A  HLA-B27 H-RRIYDLIEL-OH   EBV EBNA 3C HLA-B27 H-YPLHEQHGM-OH  EBV EBNA 3A  HLA-B35 H-IPSINVHHY-OH   CMV pp65 HLA-B35 H-EENLLDFVRF-OH   EBV HLA-B44 H-EFFWDANDIY-OH  HCMV  HLA-B44 H-TPRVTGGGAM-OH   HCMV HLA-B7 For more information and application examples, read our blog posts: "Esophagogastric adenocarcinoma: Tumor-associated antigens, immune responses and HLA-I expression" "Quality control of peptide pools in personalized cancer therapy"

This peptide pool consists of 14 peptides, each corresponding to a defined HLA class I-restricted T cell epitope from cytomegalovirus (CMV).

This peptide pool consists of 26 peptides, each corresponding to a defined HLA class I-restricted T cell epitope from Epstein-Barr virus (EBV). One unit allows the stimulation of 2,5 x 108 cells.

This peptide pool consists of 9 peptides, each corresponding to a defined HLA class I-restricted T cell epitope from Hepatitis B virus (HBV).

Peptide pool of 120 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through 55 kDa immediate-early protein 1 (IE-1) (UniProt ID: P13202) of Human cytomegalovirus (HHV-5) frequently used as positive control One unit allows the stimulation of 2,5 x 108 cells.

Peptide pool of 143 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through 45 kDa immediate-early protein 2 (IE-2) (UniProt ID: P19893) of Human cytomegalovirus (HHV-5) frequently used as positive control One unit allows the stimulation of 2,5 x 108 cells.

This peptide pool consists of 21 peptides, each corresponding to a defined HLA class I-restricted T cell epitope from Hepatitis C virus (HCV).

The peptides of this product are supplied as trifluoracetate salts. This pool consists of 22 peptides, each corresponding to a defined HLA class I-restricted T cell epitope from human immunodeficiency virus (HIV). One unit allows the stimulation of 2,5 x 108 cells.

The HUMAN (Actin) Peptide Pool consists of 92 overlapping peptides derived from a peptide scan through Actin, alpha skeletal muscle (UniProt ID: P68133) of Homo sapiens. The peptides are 15mers that overlap by 11 amino acids. One unit of HUMAN (Actin) Peptide Pool allows the stimulation of 2,5 x 108 cells. Actin is a ubiquitously expressed protein and therefore an excellent negative control for T cell assays. Relevant controls are critical for measuring antigen-specific responses as they allow accurate interpretation of test results. The HUMAN (Actin) Peptide Pool can rule out non-specific binding of peptides to the tested T cells, while a negative control with medium detects general background signals. Examples of publications where actin was used as a negative control: Baker FL, et al.: Exercise mobilizes diverse antigen specific T-cells and elevates neutralizing antibodies in humans with natural immunity to SARS CoV-2. Brain Behav Immun Health. 2023 Mar;28:100600. doi: 10.1016/j.bbih.2023.100600. Sweeney, E et al.: Photothermal Prussian blue nanoparticles generate potent multi‐targeted tumor‐specific T cells as an adoptive cell therapy. Bioeng Transl Med. 2023;e10639. doi.org/10.1002/btm2.10639. Roy, P et al.: Immunodominant MHC-II (Major Histocompatibility Complex II) Restricted Epitopes in Human Apolipoprotein B. Circ Res. 2022 Jul 22;131(3):258-276. doi: 10.1161/CIRCRESAHA.122.321116. Lasagna, Angioletta et al. “The immunogenicity and the safety of the adjuvanted glycoprotein E (gE)-based recombinant vaccine against herpes zoster (RZV) in cancer patients during immunotherapy.”Human vaccines & immunotherapeutics vol. 19,3 (2023): 2288282. doi:10.1080/21645515.2023.2288282

Pool of 45 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through ancer/testis antigen family 45 member A1 (Uniprot ID: Q5HYN5) of Homo sapiens (Human) for T cell assays. One unit allows the stimulation of 2,5 x 108 cells.

Control Pool of 172 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through Testis-specific gene 10 protein (UniProtKB - P06725) of homo sapiens. One unit allows the stimulation of 2,5 x 108 cells.

This pool consists of 17 peptides, each corresponding to a defined HLA class I-restricted T cell epitope from influenza. One unit allows the stimulation of 2,5 x 108 cells.

OVA-Peptid (323-339) is a linear peptidic epitope (epitope ID 28676) with the amino acid sequence ISQAVHAAHAEINEAGR. The peptide is studied as part of Ovalbumin (UniProt:P01012) from chicken. It is a MHC-II binding epitope, stimulating ovalbumin-specific CD4+ T cells. OVA-Peptid (323-339) serves as a negative control, when investigating CD4+ T-cell responses.

This pool consists of 28 peptides, each corresponding to a defined HLA class I-restricted T cell epitope from Respiratory Syncytial Virus (RSV).

Peptide pool of 326 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through Tetanus toxin (UniProt ID: P04958) of Clostridium tetani (strain Massachusetts / E88) for T cell assays (e.g. ELISPOT) One unit allows the stimulation of 2,5 x 108 cells.