EBV EBNA2 280-290 Peptide TVFYNIPPMPL

The EBV (EBNA-3a) Peptide Pool from peptides&elephants is designed for in vitro T cell stimulation and epitope analysis in research on Epstein-Barr virus (EBV, HHV-4). This pool contains 234 synthetic 15-mer peptides, overlapping by 11 amino acids, and spans the complete sequence of EBNA-3a (Epstein-Barr nuclear antigen 3a; UniProt ID: P12977). Each unit enables the stimulation of up to 2.5 × 10⁸ peripheral blood mononuclear cells (PBMCs) and is ideal for use in ELISpot, intracellular cytokine staining (ICS), and flow cytometry-based T cell assays. Scientific Background EBV is a ubiquitous herpesvirus that infects more than 90% of the global population. It is the causative agent of infectious mononucleosis and is closely associated with several B cell malignancies, including post-transplant lymphoproliferative disease (PTLD), diffuse large B cell lymphoma (DLBCL), Burkitt lymphoma, and Hodgkin lymphoma. During latency, EBV can enter different gene expression programs. Latency type III, in which all nine latent proteins are expressed, is the only form capable of transforming B cells in vitro, resulting in lymphoblastoid cell lines (LCLs). Among these latency proteins, EBNA-3a plays a supportive but non-essential role in B cell transformation: EBV mutants lacking EBNA-3a can still transform B cells, but with significantly reduced efficiency. EBNA-3a–deficient LCLs proliferate more slowly and show increased apoptosis compared to wild-type virus–derived LCLs. EBNA-3a appears to regulate a subset of cellular genes, some of which overlap with those regulated by EBNA2. The two proteins can act antagonistically or cooperatively to control gene expression. These findings position EBNA-3a as a key modulator of B cell proliferation and survival during EBV latency, and as a relevant CD8⁺ T cell target in both basic immunological studies and translational research.

Peptide pool of 234 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through Epstein-Barr nuclear antigen 4 (EBNA3b) (UniProt ID: Q1HVG4) of Epstein-Barr virus (HHV4) for T cell assays. One unit allows the stimulation of 2,5 x 108 cells.

The EBV EBNA-3B 399–408 peptide (AVFDRKSDAK), historically also known as EBNA-4 399–408, is one of the most thoroughly validated CD8+ T-cell targets derived from Epstein-Barr nuclear antigen 3B (EBNA-3B / EBNA-4; UniProt: P03203; epitope ID: 5316) of Epstein-Barr Virus (EBV / Human Herpesvirus 4). Expressed during latent phase III infection, EBNA-3B triggers potent cytotoxic T-lymphocyte (CTL) responses. Primary presentation occurs via HLA-A*11:01, though functional literature also confirms presentation via HLA-A*03:01, providing high population coverage for human immunomonitoring assays.Key Features & BenefitsDual HLA-A Supertype Recognition: Primarily restricted by HLA-A*11:01 and cross-validated for presentation on HLA-A*03:01, enabling broad applicability across diverse demographic cohorts.Exceptionally Benchmark-Validated: Supported by an extensive evidence base of 41 peer-reviewed publications, 80 functional T-cell assays, and 13 MHC ligand binding studies.Core Control Component: Integrated as a key reference constituent in peptides&elephants' EBV Control Peptide Pool and CEF (Advanced) Peptide Pool (>95% HPLC purity).ApplicationsEBV Latent Phase Immunomonitoring: Quantifies functional cytotoxic CD8+ T-cell memory (IFN-gamma, TNF-alpha, CD107a) directed against latent viral targets in human PBMCs.Recall Controls & Assay Standardization: Serves as a gold-standard positive control in ELISpot, Fluorospot, and Intracellular Cytokine Staining (ICS) assays.MHC Multimer & Tetramer Staining: Ideal reference ligand for generating HLA-A*11:01 and HLA-A*03:01 peptide-MHC complexes to isolate and enumerate antigen-specific CD8+ T cells.

The Epstein-Barr virus (EBV), also called Human herpes virus 4 (HHV-4), is a virus of the herpes family (which includes Herpes simplex virus and Cytomegalo virus). On infecting the B-lymphocyte, the linear virus genome circularizes and the virus subsequently persists within the cell as an episome. The virus can execute several distinct programs of gene expression which can be broadly categorized as being lytic cycle or latent cycle. The lytic cycle or productive infection results in staged expression of a host of viral proteins with the ultimate objective of producing infectious virions. Formally, this phase of infection does not inevitably lead to lysis of the host cell as EBV virions are produced by budding from the infected cell. The latent cycle (lysogenic) programs are those that do not result in production of virions.

Pool of 158 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through Epstein-Barr nuclear antigen 1 (EBNA1) (Uniprot ID: P03211) of Epstein-Barr virus (HHV4) of Epstein-Barr virus (HHV4) for T cell assays. One unit allows the stimulation of 2,5 x 108 cells.

The EBV EBNA-2 Peptide Pool from peptides&elephants is designed for reliable stimulation and detection of EBV-specific T-cell responses in immunological research. The pool consists of 119 overlapping peptides (15-mers with an 11-amino-acid overlap) spanning Epstein–Barr nuclear antigen 2 (EBNA2) from Epstein–Barr virus strain B95-8 (UniProt ID: P12978). The Epstein-Barr virus is also known as Human herpesvirus 4 (HHV-4).  EBNA2 is one of the earliest viral proteins expressed after infection of B cells and acts as a central transcriptional regulator that reprograms host and viral gene expression. Through interactions with numerous sites in the host genome, EBNA2 cooperates with EBNA-LP to activate key cellular pathways, including transient activation of the MYC proto-oncogene, which drives early B-cell proliferation and supports survival during the establishment of EBV latency. EBNA2 activity also contributes to the transition from the initial Wp promoter–driven transcription toward the Cp promoter–controlled latency program IIb, in which multiple EBV nuclear antigens are expressed from a polycistronic transcript. This shift allows the virus to balance rapid proliferation with controlled persistence. Learn more about EBNA2-mediated transcriptional reprogramming and MYC regulation during early EBV infection in the Peptide Insights section of peptides&elephants. Applications Suitable for multiple T-cell–based assays, including: ELISpot assays Intracellular cytokine staining (ICS) FluoroSpot assays T-cell proliferation assays Immune monitoring in EBV research Capacity One unit allows the stimulation of 2.5 × 10⁸ cells. The EBV EBNA-2 Peptide Pool provides a comprehensive tool for studying EBV-specific immune responses, latency establishment, and virus-host interactions in basic and translational research.

Antigen Peptide EBV EBNA3A HLA-B*08:01 (FLRGRAYGL) for stimulation of human EBV EBNA-3A(325-333) -specific CD8+ T-cells. The peptide is synthesised as it is presented by the MHC class I HLA-B*0801 allele.

EBNA3C-derived peptide of EENLLDFVRF covering 281-290 and B*44:05 molecule. The EBV EBNA3C 281-290 (HLA-B*44:05) peptide recognizes CD8 T cells, and can be used in the analysis of individual antigen-specific T cells. EENLLDFVRF is a linear peptidic epitope (epitope ID11804) studied as part of Epstein-Barr nuclear antigen 6 from Human herpesvirus 4 (Epstein Barr virus). This epitope has been studied for immune reactivity, tested in T cell assays and MHC ligand assays