This is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide from ovalbumin presented by the class I MHC molecule, H-2Kb. Ovalbumin peptide SIINFEKL (H-2 Kb) for stimulation of T cells. Single peptide (SIINFEKL) for stimulation of mouse Ovalbumin-specific CD8+ T cells. The peptide is synthesised as it is presented by the MHC class I H-2 Kb allele.
The HUMAN (Actin) Peptide Pool consists of 92 overlapping peptides derived from a peptide scan through Actin, alpha skeletal muscle (UniProt ID: P68133) of Homo sapiens. The peptides are 15mers that overlap by 11 amino acids.
One unit of HUMAN (Actin) Peptide Pool allows the stimulation of 2,5 x 108 cells.
Actin is a ubiquitously expressed protein and therefore an excellent negative control for T cell assays. Relevant controls are critical for measuring antigen-specific responses as they allow accurate interpretation of test results. The HUMAN (Actin) Peptide Pool can rule out non-specific binding of peptides to the tested T cells, while a negative control with medium detects general background signals.
Examples of publications where actin was used as a negative control:
Baker FL, et al.: Exercise mobilizes diverse antigen specific T-cells and elevates neutralizing antibodies in humans with natural immunity to SARS CoV-2. Brain Behav Immun Health. 2023 Mar;28:100600. doi: 10.1016/j.bbih.2023.100600.
Sweeney, E et al.: Photothermal Prussian blue nanoparticles generate potent multi‐targeted tumor‐specific T cells as an adoptive cell therapy. Bioeng Transl Med. 2023;e10639. doi.org/10.1002/btm2.10639.
Roy, P et al.: Immunodominant MHC-II (Major Histocompatibility Complex II) Restricted Epitopes in Human Apolipoprotein B. Circ Res. 2022 Jul 22;131(3):258-276. doi: 10.1161/CIRCRESAHA.122.321116.
Lasagna, Angioletta et al. “The immunogenicity and the safety of the adjuvanted glycoprotein E (gE)-based recombinant vaccine against herpes zoster (RZV) in cancer patients during immunotherapy.”Human vaccines & immunotherapeutics vol. 19,3 (2023): 2288282. doi:10.1080/21645515.2023.2288282
The linear peptide B8R 20-27, with the amino acid sequence TSYKFESV (epitope ID 66504), is a well-established H2-Kb-restricted epitope conserved among various Vaccinia virus strains. [1] It is part of the:
Soluble interferon gamma receptor OPG193 (UniProt:P24770) from Vaccinia virus
IFN-gamma receptor (UniProt:Q66793) from Ectromelia mousepox virus
IFN-gamma receptor-like protein (UniProt:G0XTP5) from Cowpox virus
The Vaccinia virus was the live vaccine used to eradicate smallpox. It belongs to the orthopoxvirus family and provides protection against a range of orthopoxviruses. Currently, the most concerning of these is the monkeypox virus, but human cowpox infections are also being reported regularly in Europe. [1]
The B8R 20-27 peptide, TSYKFESV, is also contained in recombinant and non-recombinant Modified Vaccinia virus Ankara (MVA). [1] The Modified Vaccinia virus Ankara has greatly reduced virulence and can no longer replicate in most mammalian cells. [2] It is often used as a vaccine vector, due to its cost-effectiveness, safety, and high immunogenicity. Recombinant MVA vaccines can generate robust humoral and cellular immune responses against various pathogens.
In vaccine development, the B8R 20-27 peptide is used as a positive control to demonstrate that a lack of response is not due to immunization failure. The vaccinia virus B8R gene encodes a homolog of the secreted IFN-γ receptor. By binding to IFN-γ, B8R neutralizes its antiviral activity and is therefore involved in virus evasion of host interferon responses. [1,3]You can read a current application example in our blog post "Vaccines against Chlamydia trachomatis: Immunogenicity analysis via peptide pool" or view the original publication [4] via the link under the References tab.
The CEF peptide pool is a lyophilized mixture of 23 peptides from cytomegalovirus (CMV), Epstein-Barr virus (EBV), and influenza virus. It consists of defined HLA class I-restricted T cell epitopes from these three viruses. The CEF peptide pool is used as a positive control to stimulate T cells. It induces secretion of e.g., IFN-γ, IL-2, and granzyme B from human CD8 T cells.
The peptides of this product are supplied as trifluoracetate salts.
One unit allows the stimulation of 2,5 x 108 cells.
The CEF peptide pool is a lyophilized mixture of 32 peptides from cytomegalovirus (CMV), Epstein-Barr virus (EBV), and influenza virus. It consists of defined HLA class I-restricted T cell epitopes from these three viruses, and can be used as a positive control to stimulate T cells. The CEF peptide pool is used as a positive control to stimulate T cells. It induces secretion of e.g., IFN-γ, IL-2, and granzyme B from human CD8 T cells.
One unit allows the stimulation of 2,5 x 108 cells.These are sequence, source and corresponding MHC class I molecule of each peptide:
Sequence
Source
MHC class I molecule
H-VSDGGPNLY-OH
Influenza A
HLA-A1
H-CTELKLSDY-OH
Influenza A
HLA-A1
H-GILGFVFTL-OH
Influenza M
HLA-A2
H-FMYSDFHFI-OH
Influenza A
HLA-A2
H-CLGGLLTMV-OH
EBV LMP2A
HLA-A2
H-GLCTLVAML-OH
EBV BMLF1
HLA-A2
H-NLVPMVATV-OH
HCMV pp65
HLA-A2
H-KTGGPIYKR-OH
Influenza NP
HLA-A68
H-RVLSFIKGTK-OH
Influenza NP
HLA-A3
H-ILRGSVAHK-OH
Influenza A
HLA-A3
H-RVRAYTYSK-OH
EBV
HLA-A3
H-RLRAEAQVK-OH
EBV
HLA-A3
H-SIIPSGPLK-OH
Influenza M
HLA-A3/A11/A6
H-AVFDRKSDAK-OH
EBV EBNA 4NP
HLA-A11
H-IVTDFSVIK-OH
EBV
HLA-A11
H-ATIGTAMYK-OH
EBV
HLA-A11
H-DYCNVLNKEF-OH
EBV RTA
HLA-A24
H-LPFDKTTVM-OH
Influenza NP
HLA-B7
H-RPPIFIRRL-OH
EBV
HLA-B7
H-ELRSRYWAI-OH
Influenza NP
HLA-B8
H-RAKFKQLL-OH
EBV B2LF-1
HLA-B8
H-FLRGRAYGL-OH
EBV EBNA 3A
HLA-B8
H-QAKWRLQTL-OH
EBV EBNA 3
HLA-B8
H-SDEEEAIVAYTL-OH
HCMV
HLA-B18
H-SRYWAIRTR-OH
Influenza NP
HLA-B27
H-ASCMGLIY-OH
Influenza A
HLA-B27
H-RRIYDLIEL-OH
EBV EBNA 3C
HLA-B27
H-YPLHEQHGM-OH
EBV EBNA 3A
HLA-B35
H-IPSINVHHY-OH
CMV pp65
HLA-B35
H-EENLLDFVRF-OH
EBV
HLA-B44
H-EFFWDANDIY-OH
HCMV
HLA-B44
H-TPRVTGGGAM-OH
HCMV
HLA-B7
For more information and application examples, read our blog posts:
"Esophagogastric adenocarcinoma: Tumor-associated antigens, immune responses and HLA-I expression"
"Quality control of peptide pools in personalized cancer therapy"
Pool of 45 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through cancer/testis antigen family 45 member A1 (Uniprot ID: Q5HYN5) of Homo sapiens (Human) for T cell assays.
One unit allows the stimulation of 2,5 x 108 cells.
This peptide pool consists of 26 peptides, each corresponding to a defined HLA class I-restricted T cell epitope from Epstein-Barr virus (EBV).
One unit allows the stimulation of 2,5 x 108 cells.
Peptide pool of 120 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through 55 kDa immediate-early protein 1 (IE-1) (UniProt ID: P13202) of Human cytomegalovirus (HHV-5) frequently used as positive control
One unit allows the stimulation of 2,5 x 108 cells.
Peptide pool of 143 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through 45 kDa immediate-early protein 2 (IE-2) (UniProt ID: P19893) of Human cytomegalovirus (HHV-5) frequently used as positive control
One unit allows the stimulation of 2,5 x 108 cells.
The peptides of this product are supplied as trifluoracetate salts. This pool consists of 22 peptides, each corresponding to a defined HLA class I-restricted T cell epitope from human immunodeficiency virus (HIV).
One unit allows the stimulation of 2,5 x 108 cells.
This pool consists of 17 peptides, each corresponding to a defined HLA class I-restricted T cell epitope from influenza.
One unit allows the stimulation of 2,5 x 108 cells.
OVA-Peptid (323-339) is a linear peptidic epitope (epitope ID 28676) with the amino acid sequence ISQAVHAAHAEINEAGR. The peptide is studied as part of Ovalbumin (UniProt:P01012) from chicken. It is a MHC-II binding epitope, stimulating ovalbumin-specific CD4+ T cells.
OVA-Peptid (323-339) serves as a negative control, when investigating CD4+ T-cell responses.
Peptide pool of 326 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through Tetanus toxin (UniProt ID: P04958) of Clostridium tetani (strain Massachusetts / E88) for T cell assays (e.g. ELISPOT)
One unit allows the stimulation of 2,5 x 108 cells.
Control peptide pool of 172 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through human Testis-specific gene 10 protein (UniProt ID Q9BZW7).
One unit allows the stimulation of 2,5 x 108 cells.
€380.70*
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