Tetanus Toxin 947-967 (HLA-DR11) FNNFTVSFWLRVPKVSASHLE
€357.30*
Description
A universal CD4+ T-cell helper epitope and promiscuous MHC Class II control for human immunomonitoring and conjugate vaccine design.
The Tetanus Toxin 947–967 peptide (FNNFTVSFWLRVPKVSASHLE, epitope ID: 17207), widely known as the P30 helper epitope, is one of the most prominent promiscuous T-cell epitopes in cellular immunology.
Derived from the heavy chain of Clostridium tetani (strain Massachusetts / E88) neurotoxin (UniProt: P04958), this 21-mer sequence represents a core CD4+ T-cell target of vaccine-induced immune memory established by widespread Tetanus Toxoid vaccination. Because of its broad binding capacity across diverse human MHC Class II molecules, P30 is an essential reference tool for PBMC recall assays, immunogenicity enhancement, and vaccine carrier design.
Key Features & Benefits
- Promiscuous HLA Class II Binding: Binds efficiently across a wide spectrum of human MHC Class II alleles, ensuring high population coverage without donor HLA restriction.
- Extensively Validated: Backed by dozens of scientific publications and validated across nearly 100 T-cell assays, B-cell assays, and MHC ligand binding studies worldwide.
- Robust Positive Control: Serves as a gold-standard recall antigen for evaluating functional CD4+ T-cell viability (IFN-gamma, IL-2, TNF-alpha) in human peripheral blood mononuclear cells (PBMCs).
- Ideal Carrier & Helper Sequence: Frequently paired or benchmarked alongside the P2 epitope (TT 830–843) to enhance the immunogenicity of synthetic peptide vaccines, neoantigens, and carbohydrate conjugates.
- High Chemical Quality: The rigorous quality standards of peptides&elephants guarantee exceptional batch-to-batch consistency, solubility, and assay reproducibility.
Applications
- PBMC Quality Control & Recall Assays: Acts as a reliable positive control in ELISpot, Fluorospot, Intracellular Cytokine Staining (ICS), and proliferation assays evaluating Tetanus Toxoid-induced T-cell memory.
- Conjugate Vaccine & Neoantigen Design: Widely incorporated into synthetic multi-epitope construct designs to provide strong, promiscuous CD4+ T-cell help for antibody or CD8+ T-cell responses.
- HLA Class II Profiling: Excellent reference target for investigating CD4+ T-cell receptor (TCR) cross-reactivity and MHC-II binding kinetics.
TechData
| Sequence: | FNNFTVSFWLRVPKVSASHLE |
| Gene: | tetX |
| Delivery: | 1-3 days |
| C-Terminus: | OH |
| N-Terminus: | H |
| Amount: | 5 mg |
| Counterion: | TFA |
| Protein: | Tetanus Toxin |
| UniProt Id: | P04958 |
| Species: | Clostridium tetani (strain Massachusetts / E88) |
| Allele: | HLA-DR11 |
| Application: | T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response |
| Indication: | Infectious disease, Neuroscience, Control |
| Purity: | 95% HPLC-MS |
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