Infectious disease

HCMV UL40 15-23 (HLA-E) VMAPRTLIL

€105.00*

Prices excl. VAT plus shipping costs

Available, delivery time: 1-3 days

sterile and endotoxin free

Delivery Format: The product is supplied freeze dried.

Purity: 95% HPLC-MS

Caution: For research use only. Not for use in humans.

Amount in mg

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Product number: EP06244_1

Description

TechData

Documents

References

Description

HCMV UL40 15-23 with the amino acid sequence VMAPRTLIL is a linear peptidic epitope (epitope ID 69921) that is an important negative regulator of natural killer cell activity. The HCMV UL40 15-23 peptide is often used as positive control.

HCMV UL40 15-23 is part of the immune evasion strategie of the Human Cytomegalovirus (HCMV). The virus downregulates HLA class Ia molecule expression in order to evade T cell recognition. However, a lack of surface HLA class Ia molecules triggers cytotoxicity of Natural Killer (NK) cells. To counteract this effect, the Cytomegalovirus promotes upregulation of HLA-E and provides the peptide HCMV UL40 15-23 (VMAPRTLIL), which mimics the leader sequence of HLA-Cw03 (VMAPRTLIL). When loaded onto HLA-E, this peptide conferres resistance to NK cell lysis via the CD94/NKG2A receptor.

HLA-E is presenting peptides derived from self-proteomic content and peptides of pathogenic origin. VMAPRTLIL is part of the following proteins of human origin:

Major histocompatibility complex, class I, C (UniProt:A0A140T9M0, A0A140T9U0, A0A0G2JH50, A0A140T912 and A0A140T9Z4)
HLA class I histocompatibility antigen C alpha chain (UniProt:Q53YP1, Q5RIP0)
HLA class I antigen (UniProt:Q5QT33)
Isoform 2 of HLA class I histocompatibility antigen, C alpha chain (UniProt:P10321-2)

See also an example comparison of our prices with the prices of other peptide manufacturers.

TechData

Sequence:	VMAPRTLIL
Gene:	UL111A
Delivery:	1-3 days
C-Terminus:	OH
N-Terminus:	H
Amount:	1 mg
Counterion:	TFA
Protein:	Viral interleukin-10 homolog
IEDB Id:	https://www.iedb.org/epitope/69921
Species:	Human cytomegalovirus (Human herpesvirus 5)
Allele:	HLA-E*
Application:	Flow Cytometry
Indication:	Infectious disease, Cancer, Neuroscience, Diabetes
Purity:	95% HPLC-MS

Documents

Storage and handling of peptides

Probing of membranes with HRP

References

Vietzen, Hannes et al. “Torque teno viruses exhaust and imprint the human immune system via the HLA-E/NKG2A axis.” Frontiers in immunology vol. 15 1447980. 4 Sep. 2024, doi:10.3389/fimmu.2024.1447980

Martín Almazán, Nerea et al. “Non-classical HLA-E restricted CMV 15-mer peptides are recognized by adaptive NK cells and induce memory responses.” Frontiers in immunology vol. 14 1230718. 21 Sep. 2023, doi:10.3389/fimmu.2023.1230718

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