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Structure of EBV EBNA-3A 379-387 (HLA-B*07:02)
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Infectious disease

EBV EBNA-3A 379-387 (HLA-B*07:02) RPPIFIRRL

Product number: EP05817_1

€ 105.00 *

Prices excl. VAT plus shipping costs
Available, delivery time: 1-3 days
sterile and endotoxin free
Delivery Format: The product is supplied freeze dried.
Purity: 95% HPLC-MS
Caution: For research use only. Not for use in humans.
Amount in mg
1
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Description

The EBV EBNA-3A 379–387 peptide (RPPIFIRRL; epitope ID: 55252) is an immunodominant, structurally defined, and widely cited CD8+ T-cell target in viral immunology. Derived from Epstein-Barr nuclear antigen 3A (EBNA-3A / EBNA-3; UniProt: P12977) of Epstein-Barr Virus (EBV / Human Herpesvirus 4), this nonamer sequence represents a critical cytotoxic target during latent viral infection. 

Featuring the amino acid sequence RPPIFIRRL, this catalog peptide is strictly presented via HLA-B*07:02—a highly prevalent allele in global populations—and serves as a primary model antigen in structural immunology with multiple published 3D crystal structures (PDB IDs: 6VMX, 5WMO).

Key Features & Benefits

HLA-B*07:02 Anchor Nonamer: Optimized for high-affinity presentation, enabling precise CD8+ cytotoxic T-cell activation, TCR profiling, and tetramer generation in HLA-B*07:02-positive donor cohorts.

Exceptionally Benchmark-Validated: Supported by an extensive evidence base of over 66 peer-reviewed publications, 157 functional T-cell assays, 29 MHC ligand binding studies, and multiple resolved crystal structures (PDB: 6VMX, 5WMO).

Core Control Component: Integrated as an essential reference constituent in peptides&elephants' EBV (EBNA-3a) Peptide Pool, CEF (Classic) Peptide Pool (>95% HPLC), and CEF (Advanced) Peptide Pool (>95% HPLC).

Applications

EBV Latent Phase Immunomonitoring: Measures functional CD8+ Effector and Effector Memory T-cell activation (IFN-gamma, TNF-alpha, CD107a) against latent nuclear viral antigens in human PBMCs.

Recall Controls & Assay Standardization: Serves as a gold-standard positive control in ELISpot, Fluorospot, and Intracellular Cytokine Staining (ICS) assays.

Structural & Biophysical Research: Ideal reference ligand for generating HLA-B*07:02 peptide-MHC complexes for TCR docking studies, structural biology, and MHC multimer staining.

TechData

Sequence: RPPIFIRRL
Gene: EBNA
Delivery: 1-3 days
C-Terminus: OH
N-Terminus: H
Amount: 1 mg
Counterion: TFA
Protein: Epstein-Barr nuclear antigen
UniProt Id: P12977
IEDB Id: 55252
Species: Epstein-Barr virus (EBV; Human herpesvirus 4)
Allele: HLA-B*07:02
Application: T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response
Indication: Infectious disease, Cancer, Neuroscience
Purity: 95% HPLC-MS

Documents

Material data sheet Safety data sheet Storage and handling of peptides Probing of membranes with HRP

References

Steven, N M et al. “Immediate early and early lytic cycle proteins are frequent targets of the Epstein-Barr virus-induced cytotoxic T cell response.” The Journal of experimental medicine vol. 185,9 (1997): 1605-17. doi:10.1084/jem.185.9.1605
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